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Genetic variability of two populations of Pseudoplatystoma reticulatum from the Upper Paraguay River Basin Genet. Mol. Biol.
Abreu,Marcia Matos de; Pereira,Luiz Henrique Garcia; Vila,Vilma Barretto; Foresti,Fausto; Oliveira,Claudio.
Catfishes of the genus Pseudoplatystoma are very important species due to both their high commercial value and their ecological role as voracious predators. They undertake lengthy migratory movements during their life-cycle, this including reproductive migration which occurs from October to December in the rainy season. In the present study, seven microsatellite loci were analyzed to access genetic variability in two samples of P. reticulatum from the Upper Paraguay Basin. The loci were highly polymorphic (mean = 7.28). According to all analysis, the two samples of P. reticulatum revealed pronounced genetic differentiation. Fst value was 0.2290, Rst value 0.1067 and AMOVA 22.90% (Fst) and 10.67% (Rst), all being highly significant (p < 0.001). The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Population genetics; Microsatellite; Homing; Population structure.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400032
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Genetic variability in Pseudoplatystoma reticulatum from a breeding program in Brazil Rev. Ciênc. Agron.
Albuquerque,Daniele Menezes; Oliveira,Carlos Antonio Lopes de; Rodriguez,Maria Del Pilar Rodriguez; Ribeiro,Ricardo Pereira.
ABSTRACT The aim of this study was to characterise genetic variability in five groups of Pseudoplatystoma reticulatum: Mato Grosso I (MT-I), Mato Grosso II (MT-II), Mato Grosso III (MT-III), Mato Grosso do Sul I (MS-I) and Mato Grosso do Sul II (MS-II), taken from a centre for genetic improvement. In order to determine the allelic frequency, observed heterozygosity (HO), inbreeding coefficients (FIS), Hardy-Weinberg equilibrium (HW-p) and genetic differentiation, eight polymorphic microsatellite loci (Pcor01, Pcor05, Pcor08, Pcor10, Ppu01, Ppu04, Ppu09 and Ppu10) were selected, in which a total of 62 alleles were observed in the individuals (n = 211). The MS-I population had the highest (Pcor08 allele 83: 0.5000) and lowest (Pcor10 allele 50: 0.1667) mean...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cachara; Inbreeding coefficient; Allelic Frequency; Population genetics; Microsatellites.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000100402
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Population structure and genetic variability of mainland and insular populations of the Neotropical water rat, Nectomys squamipes (Rodentia, Sigmodontinae) Genet. Mol. Biol.
Almeida,Francisca C.; Maroja,Luana S.; Moreira,Miguel A.M.; Seuánez,Héctor N.; Cerqueira,Rui.
Seven microsatellite loci were used to investigate the genetic variability and structure of six mainland and two island populations of the Neotropical water rat Nectomys squamipes, a South American semi-aquatic rodent species with a wide distribution. High levels of variability were found within mainland populations while island populations were less variable but the more differentiated in respect to allele number and frequency. The time of biological divergence between mainland and island populations coincided with geological data. A significant geographic structure was found in mainland populations (theta = 0.099; rho = 0.086) although the degree of differentiation was relatively low in respect to the distance between surveyed localities (24 to 740 km)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Population genetics; Islands; Population expansion; Population size; Divergence time.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500008
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Genetic structure at different spatial scales in the pearl oyster (Pinctada margaritifera cumingii) in French Polynesian lagoons: beware of sampling strategy and genetic patchiness ArchiMer
Arnaud, Sophie; Vonau, Vincent; Rouxel, Catherine; Bonhomme, Francois; Prou, Jean; Goyard, Emmanuel; Boudry, Pierre.
In order to study further the genetic structure of the pearl oyster Pinctada margaritifera in French Polynesia with a special consideration for the sampling scale, we analyzed or re-analyzed sets of data based on nuclear DNA markers obtained at different spatial scales. At a large scale (several 1,000 km), the remote Marquesas Islands were confirmed to be significantly differentiated from Tuamotu-Gambier and Society archipelagos, with a marked difference however for the two main islands that are different from each other. At a medium scale (several 10 to several 100 km), overall homogeneity was observed within and between these two archipelagos, with some exceptions. This could be attributed both to large-scale larval dispersal and to human-driven spat...
Tipo: Text Palavras-chave: Population genetics; Polynesia; Bivalve; Pearl oyster; Benthic organisms; Sampling strategy; Genetic patchiness.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4960.pdf
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Invasion of the eastern Bay of Biscay by the nassariid gastropod Cyclope neritea: origin and effects on resident fauna ArchiMer
Bachelet, G; Simon-bouhet, B; Desclaux, C; Garcia-meunier, P; Mairesse, G; De Montaudouin, X; Raigne, H; Randriambao, K; Sauriau, Pierre-guy; Viard, F.
The distribution area of the nassariid gastropod Cyclope neritea (L.) includes the Mediterranean and the Black Sea, as well as the Atlantic coasts of the southern Iberian Peninsula. The species has spread north to the eastern Bay of Biscay (Arcachon Bay in 1976, Marennes-Oleron area and Morbihan Gulf in 1983-84). This spread might be explained either by (1) a natural spread favoured by environmental changes (e.g. an increase of temperature) or (2) a sudden range expansion due to the introduction of individuals from distant native populations. Molecular genetic analyses based on mitochondrial markers suggest that the present C. neritea population in Arcachon Bay has been introduced, probably unintentionally with oyster transfers, from several source...
Tipo: Text Palavras-chave: Introduced species; Scavenging gastropods; Population genetics; Interspecific competition; Behaviour parasites.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00000/10848/7582.pdf
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Genetic diversity and relationship in American and African oil palm as revealed by RFLP and AFLP molecular markers PAB
Barcelos,Edson; Amblard,Philippe; Berthaud,Julien; Seguin,Marc.
The objective of this work was to evaluate the genetic diversity, its organization and the genetic relationships within oil palm (Elaeis oleifera (Kunth) Cortés, from America, and E. guineensis (Jacq.), from Africa) germplasm using Restriction Fragment Length Polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP). In complement to a previous RFLP study on 241 E. oleifera accessions, 38 E. guineensis accessions were analyzed using the same 37 cDNA probes. These accessions covered a large part of the geographical distribution areas of these species in America and Africa. In addition, AFLP analysis was performed on a sub-set of 40 accessions of E. oleifera and 22 of E. guineensis using three pairs of enzyme/primer combinations. Data were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Elaeis oleifera; Elaeis guineensis; Population genetics; Palm species; Genetic resources; Molecular chacterization.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002000800008
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Estimates of genetic parameters and variability in provenances and progeny of Handroanthus vellosoi PFB - Pesquisa Florestal Brasileira
Batista, Camila Moreira; Freitas, Miguel Luiz Menezes; Moraes, Marcela Aparecida de; Zanatto, Antonio Carlos Scatena; Santos, Pedro César dos; Zanata, Marcelo; Moraes, Mario Luiz Teixeira de; Sebbenn, Alexandre Magno.
The present study aimed at estimating genetic parameters and variability among and within provenances for silvicultural traits of Handroanthus vellosoi (Toledo) Mattos, in provenances and progenies test stands, located at the Luiz Antônio Experimental Station (São Paulo State, Brazil). The test was established in the compact family block design, with two provenances (allocated in the plots), six replicates and sub-linear plots of five plants, totalizing 35 progenies. Diameter at breast height (DBH), plant height, cylindrical volume, stem form and survival at 24 years of age were evaluated. Significant differences among the provenances were observed only to the trait stem form. The analysis of deviance showed significant differences among progenies for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ex situ conservation; Tropical tree species; Population genetics; Quantitative genetics Recursos genéticos florestais; Conservação genética; Genética de populações; Genética quantitativa; Melhoramento genético florestal Conservação ex situ; Espécies arbóreas tropicais; Genética de populações; Genética quantitativa.
Ano: 2012 URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/358
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Heredabilidad y correlaciones genéticas y fenotípicas para caracteres de crecimiento en el camarón blanco Litopenaeus schmitti (Burkenroad, 1936), (Decapoda, Dendrobranchiata). OceanDocs
Bécquer Zuñiga, U..
En Cuba el cultivo de camarÿn marino Litopenaeus schmitti ha sido una actividad priorizada por el Estado, por la cantidad de empleos que genera y los ingresos obtenidos por la comercializaciÿn del producto final. Trabajos previos indican el conocimiento de la estructura genþtica de las poblaciones de esta especie empleando marcadores moleculares, existen estudios sobre reproducciÿn y nutriciÿn. Finalmente, el cierre del ciclo de cultivo en cautiverio sumado a la experiencia de casi dos dþcadas en esta actividad promueven la necesidad de comenzar el mejoramiento genþtico de L. schmitti por crýas selectivas en el paýs. En este trabajo se describe una metodologýa para la formaciÿn de grupos genþticos por...
Tipo: Theses and Dissertations Palavras-chave: Shrimp culture; Genetic selection; Population genetics.
Ano: 2005 URL: http://hdl.handle.net/1834/5399
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Polymorphisme intraspécifique chez le syngnathe aiguille, <Syngnathus acus> (osteichthyes, syngnathidae) des eaux Tunisennes (Méditérranée centrale) OceanDocs
Ben Amor, M. M.; Ben Salem, M.; Capapé, C..
De juin 2002 à juin 2005, 267 syngnathes aiguilles, <Syngnathus acus> Linnaeus, 1758, ont été récoltés dans les eaux tunisiennes: 204 dans Bahiret El Biban (Tunisie méridionale), 24 dans la lagune Sud de Tunis et 39 devant La Goulette (Tunisie septentrionale). Trois types d'études ont été effectués à partir de l’ensemble des variables biométriques: la première repose sur l'analyse de la variance et de la moyenne à l'aide du test "F" de Ficher et du test "t" de Student; la deuxième est fondée sur l’analyse de la multivariance (ANOVA); et la troisième est fondée sur des analyses multidimensionnelles. La première et la deuxième études ont montré des différences significatives dans les variances et les moyennes pour la plupart des caractères examinés,...
Tipo: Journal Contribution Palavras-chave: Biometrics; Biopolymorphism; Population genetics; Statistical analysis.
Ano: 2008 URL: http://hdl.handle.net/1834/4234
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When physical oceanography meets population genetics: The case study of the genetic/evolutionary discontinuity in the endangered goliath grouper (Epinephelus itajara, Perciformes: Epinephelidae) with comments on the conservation of the species ArchiMer
Benevides, E. A.; Vallinoto, M. N. S.; Fetter Filho, A. F. H.; De Souza, J. R. B.; Silva-oliveira, G.; Freitas, M. O.; Ferreira, B. P.; Hostim-silva, M.; Bertoncini, A. A.; Blanchard, Fabian; Torres, R. A..
Epinephelus itajara is one of the marine fish species most threatened for extinction and it is considered to be "critically endangered" by the IUCN. The present study evaluated the genetic diversity of the species and the genetic/evolutionary relationships of its populations along the Atlantic coast of South America. The results indicate relatively reduced genetic variation, re-emphasizing the low adaptive potential of the species. One of the populations presented relatively high degrees of genetic diversity and it is evolutionary isolated from the all other populations. The evidences indicate the existence of two Evolutionarily Significant Units comprising E. itajara in the Atlantic coast of South America and the conservation prospects for the species...
Tipo: Text Palavras-chave: Epinephelus itajara; ISSRs; Population genetics; Conservation genetics.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00230/34100/34465.pdf
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Genetic diversity and population structure of bocachico Prochilodus magdalenae (Pisces, Prochilodontidae) in the Magdalena River basin and its tributaries, Colombia Genet. Mol. Biol.
Berdugo,Gilberto Orozco; Narváez Barandica,Juan C..
Prochilodus magdalenae is an endemic freshwater fish that occurs in the Magdalena, Sinú and Atrato hydrographic basins. It has an important economic role and is a food resource for the artisanal fishing communities. Its socioeconomic importance contrasts with the current status of its fisheries, where stocks are being depleted. Considering its importance and lack of information on its genetic structure, we used seven microsatellite markers to assess the genetic structure of wild populations of P. magdalenae. The genetic diversity was assessed and the population genetic structure was estimated through Fst, analysis of molecular variance and Bayesian analysis. A total of 290 alleles were found in all loci throughout all population. The high polymorphism...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neotropical fish; Microsatellite; Conservation genetics; Population genetics.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100008
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Gene variation and genetic differentiation among populations of the solitary mud dauber wasp Trypoxylon (Trypargilum) albitarse Fabricius 1804 (Hymenoptera, Crabronidae) Genet. Mol. Biol.
Bergamaschi,Antonio C.B.; Lama,Marco A. Del.
Abstract Trypoxylon is a genus of solitary crabronid wasps whose population genetics is poorly known. The purpose of the present study was to investigate the genetic variation and differentiation among five populations of Trypoxylon albitarse, a species widely distributed throughout the Neotropics, with records from Panama to northern Argentina. Eight species-specific microsatellite loci were used for genotyping 96 adult wasps (one female per nest) sampled at five sites in Brazil. The analysis of allelic richness and private alleles indicated high genetic diversity in the populations sampled. Pairwise comparisons using the Fst and Dest indices revealed significant differentiation for all, but one pair of populations. Fst, Dest, AMOVA and assignment test...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Population genetics; Wasps; Crabronidae; SSR markers.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400519
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Mark-recapture cloning: a straightforward and cost-effective cloning method for population genetics of single-copy nuclear DNA sequences in diploids ArchiMer
Bierne, Nicolas; Tanguy, A; Faure, Michel; Faure, B; David, E; Boutet, I; Boon, E; Quere, N; Plouviez, S; Kemppainen, P; Jollivet, D; Moraga, D; Boudry, Pierre; David, P.
We describe a simple protocol to reduce the number of cloning reactions of nuclear DNA sequences in population genetic studies of diploid organisms. Cloning is a necessary step to obtain correct haplotypes in such organisms, and, while traditional methods are efficient at cloning together many genes of a single individual, population geneticists rather need to clone the same locus in many individuals. Our method consists of marking individual sequences during the polymerase chain reaction (PCR) using 5'-tailed primers with small polynucleotide tags. PCR products are mixed together before the cloning reaction and clones are sequenced with universal plasmid primers. The individual from which a sequence comes from is identified by the tag sequences upstream...
Tipo: Text Palavras-chave: Sequence; Population genetics; High throughput allele recognition; DNA polymorphism.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4222.pdf
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Populational genetic structure and sociogenetic structure of cocoon masses of Digelasinus diversipes (Kirby, 1882) (Hymenoptera: Symphyta: Argidae) Genet. Mol. Biol.
Boraschi,Daniele; Del Lama,Marco Antonio.
Gene variation and population genetic structure of the Neotropical sawfly Digelasinus diversipes were measured by allozyme analyses using starch gel electrophoresis. Cocoon masses were collected in Eugenia glazioviana (Myrtaceae) stems, in two areas of the "Estação Ecológica Jataí" (Luiz Antônio, SP, Brazil - 21°25' S, 47°50' W), in 2000 and 2001. The average heterozygosity observed in this species (Hobs = 0.094 ± 0.025) was not significantly different from other Symphyta groups; it was, however, significantly higher than in other Hymenoptera populations. No significant levels of inbreeding were found (F IS = 0.062; chi2 = 29.9; p > 0.05), but the population was subdivided (F ST = 0.070; chi2 = 458.9; p < 0.05), suggesting the absence of a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; Population genetics; Digelasinus diversipes; Neotropical sawfly; Allozymes.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300013
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An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine Estuary ArchiMer
Borcier, Elodie; Charrier, Gregory; Couteau, Jérôme; Maillet, Géraldine; Le Grand, Fabienne; Bideau, Antoine; Waeles, Mathieu; Le Floch, Stephane; Amara, Rachid; Pichereau, Vianney; Laroche, Jean.
The objective of this study was to develop an integrative approach in ecotoxicology (from biomarkers to population genetics) to assess the ecological status of fish populations. Flounders (Platichthys flesus) collected after the spawning season in the heavily polluted Seine estuary were compared with the moderately polluted Bay of Douarnenez. The muscle energetic reserves were highly depleted in Seine vs. Douarnenez fish. The Seine fish displaying a reduced capacity to manage the oxidative stress and a higher energetic metabolism. An increase in the content of muscle membrane phospholipids (sphingomyelin, phosphatidylserine, free sterols) was detected in the Seine vs. Douarnenez fish. The data integration allowed to hypothesize relationships between...
Tipo: Text Palavras-chave: Platichthys flesus; Estuaries; Biomarkers; Bioenergetics; Polar lipids; Population genetics.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00658/76981/78238.pdf
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Genetic variability in five populations of Partamona helleri (Hymenoptera, Apidae) from Minas Gerais State, Brazil Genet. Mol. Biol.
Borges,Andreia Arantes; Campos,Lucio Antônio de Oliveira; Salomão,Tânia Maria Fernandes; Tavares,Mara Garcia.
Partamona is a Neotropical genus of stingless bees that comprises 33 species distributed from Mexico to southern Brazil. These bees are well-adapted to anthropic environments and build their nests in several substrates. In this study, 66 colonies of Partamona helleri from five localities in the Brazilian state of Minas Gerais (São Miguel do Anta, Teixeiras, Porto Firme, Viçosa and Rio Vermelho) were analyzed using nine microsatellite loci in order to assess their genetic variability. Low levels of observed (Ho = 0.099-0.137) and expected (He = 0.128-0.145) heterozygosity were encountered and revealed discrete genetic differentiation among the populations (F ST =0.025). AMOVA further showed that most of the total genetic variation (94.24%) in P. helleri was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellites; Partamona helleri; Population genetics; Stingless bees.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400028
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Diversité et différentiation génétique chez l'huître plate européenne : de la population à l'individu (Actes) ArchiMer
Boudry, Pierre; Launey, Sophie; Diaz-almela, A.; Naciri-graven, Yamama; Ledu, Christophe; Mira, S.; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
PCR-based DNA markers enable the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European fiat oyster (Ostrea edulis) populations aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Similar patterns of variation were found using allozymes, microsatellites and mitochondrial DNA, aIl which support an isolation by distance model. High variability was observed in the range of diversity between populations using a mitochondrial marker, a reflection of small effective population sizes in sorne locations. A 10fold quantitative difference was observed in Fst,...
Tipo: Text Palavras-chave: Recruitment; Diversity; Molecular markers; Population genetics; Ostrea edulis; Flat oyster; Dynamique de recrutement; Diversité génétique; Microsatellites; Marqueurs moléculaires; Génétique des populations; Ostrea edulis; Huître plate.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-2851.pdf
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Population genetics of the European flat oyster (Ostrea edulis) : from larvae to populations ArchiMer
Boudry, Pierre; Launey, Sophie; Diaz-almela, E.; Naciri-graven, Yamama; Ledu, Christophe; Mira, Sara; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
Nowadays, PCR-based DNA markers allow the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European flat oyster (Ostrea edulis) populations has aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Results were congruent between allozymes, microsatellites and mitochondrial DNA and support an isolation by distance model. High variability was observed in the breadth of diversity between populations using a mitochondrial marker, this highlights the small effective population sizes in some locations. A 10-fold quantitative difference was observed in Fst...
Tipo: Text Palavras-chave: Larvae; Reproduction; Genetic; Ostrea edulis; European flat oyster; Population genetics.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/acte-3493.pdf
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Population genetic structuring in pacu (Piaractus mesopotamicus) across the Paraná-Paraguay basin: evidence from microsatellites Neotropical Ichthyology
Calcagnotto,Daniela; DeSalle,Rob.
The Paraná-Paraguay basin encompasses central western Brazil, northeastern Paraguay, eastern Bolivia and northern Argentina. The Pantanal is a flooded plain with marked dry and rainy seasons that, due to its soil characteristics and low declivity, has a great water holding capacity supporting abundant fish fauna. Piaractus mesopotamicus, or pacu, endemic of the Paraná-Paraguay basin, is a migratory species economically important in fisheries and ecologically as a potential seed disperser. In this paper we employ eight microsatellite loci to assess the population structure of 120 pacu sampled inside and outside the Pantanal of Mato Grosso. Our main objective was to test the null hypothesis of panmixia and to verify if there was a different structuring...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neotropical fish; Pantanal of Mato Grosso; Population genetics; Migratory fish; Serrasalmidae.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400008
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Isoenzymatic polymorphism in the leaf-cutting ant Atta capiguara Gonçalves (hymenoptera: formicidae) Neotropical Entomology
Cantagalli,Liriana B; Mangolin,Claudete A; Ruvolo-Takasusuki,Maria C C.
This study was carried out to analyze the genetic population structure of Atta capiguara from 12 nests collected in Tapejara in the state of Paraná, Brazil, using isoenzyme polymorphisms. The analyzed isoenzymes were esterases (EST - EC 3.1.1.1), acid phosphatase (ACP - EC 3.1.3.2) and carbonic anhydrase (CA - EC 4.2.1.1). Ten loci were found in A.capiguara and four polymorphic loci were detected. The observed heterozigosity (0.0296) was low when compared to the expected heterozigosity (0.1461). The high value of F IS (0.7954) shows an excess of homozygous genotypes probably caused by inbreeding.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isoenzyme; Genetic variability; Population genetics.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000100007
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